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Ca 2+ ‐regulated cell migration revealed by optogenetically engineered Ca 2+ oscillations
Author(s) -
Lai YiShyun,
Chang YaHan,
Chen YongYi,
Xu Jixuan,
Yu ChiSian,
Chang SuJing,
Chen PaiSheng,
Tsai ShawJenq,
Chiu WenTai
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.30190
Subject(s) - optogenetics , intracellular , microbiology and biotechnology , cell , biophysics , stimulation , chemistry , transcription factor , positive feedback , biology , biochemistry , neuroscience , gene , engineering , electrical engineering
The ability of a single Ca 2+ ion to play an important role in cell biology is highlighted by the need for cells to form Ca 2+ signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca 2+ concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca 2+ translocating channelrhodopsin (CatCh) were used to mediate Ca 2+ influx through blue light illumination with various parameters, such as intensity, frequency, duty cycle, and duration. We identified that several Ca 2+ ‐dependent transcription factors and certain kinases can be activated by specific Ca 2+ waves. Using a wound‐healing assay, we found that low‐frequency Ca 2+ oscillations increased cell migration through the activation of NF‐κB. This study explores the regulation of cell migration by Ca 2+ signals. Thus, we can choose optical parameters to modulate Ca 2+ waves and achieve activation of specific signaling pathways. This novel methodology can be applied to clarify related cell‐signaling mechanisms in the future.