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Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer
Author(s) -
GutiérrezCastañeda Luz D.,
TovarParra David,
Quintero Gloria,
Amezquita Lorena,
Guerrero Carlos,
Sanabria Daniel
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29220
Subject(s) - cd44 , vimentin , ovarian cancer , phenotype , mesenchymal stem cell , biology , epithelial–mesenchymal transition , cancer research , immunofluorescence , antibody , cancer , ovarian tumor , pathology , cell , immunohistochemistry , immunology , medicine , metastasis , gene , genetics
Ovarian cancer is the fifth leading cause of cancer‐related deaths. It causes approximately 125,000 deaths per year worldwide; its diagnosis is made in advanced stages resulting in a high mortality rate. The objective of the study was optimizing the isolation of cells obtained from the solid tumor and ascitic fluid of patients with ovarian cancer and the phenotype with markers related to the epithelial–mesenchymal transition. For this, the solid tumor tissue was disaggregated and cultivated with different methodologies. As a result, cell growth was obtained and epi‐immunofluorescence was performed using antibodies against E‐cadherin, EpCAM, N‐cadherin, vimentin, CD133, and CD44. The primary culture from the solid tumor was obtained using Dispase II and DMEM/F12. Finally, heterogeneity was detected in terms of the expression of mesenchymal and epithelial type markers in the two types of isolated cells. Additionally, CD133 and CD44 expression was detected, proteins associated with the tumor stem cells phenotype.