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Organic solvent‐based tissue clearing techniques and their applications
Author(s) -
Zhan Yanjing,
Wu Haoyan,
Liu Linfeng,
Lin Jie,
Zhang Shiwen
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000413
Subject(s) - clearing , slicing , biochemical engineering , computer science , organic solvent , process engineering , solvent , biomedical engineering , materials science , nanotechnology , chemistry , medicine , chemical engineering , engineering , business , organic chemistry , world wide web , finance
Abstract Revealing the true structure of tissues and organs with tissue slicing technology is difficult since images reconstructed in three dimensions are easily distorted. To address the limitations in tissue slicing technology, tissue clearing has been invented and has recently achieved significant progress in three‐dimensional imaging. Currently, this technology can mainly be divided into two types: aqueous clearing methods and solvent‐based clearing methods. As one of the important parts of this technology, organic solvent‐based tissue clearing techniques have been widely applied because of their efficient clearing speed and high clearing intensity. This review introduces the primary organic solvent‐based tissue clearing techniques and their applications.