Premium
The Role of Kupffer Cells After Major Liver Surgery
Author(s) -
Prins Hubert A.,
Meijer Catharina,
Boelens Petra G.,
Nijveldt Robert J.,
Siroen Michiel P. C.,
Masson Sylvie,
Daveau Maryvonne,
Scotté Michel,
Diks Jeroen,
Leeuwen Paul A. M.
Publication year - 2005
Publication title -
journal of parenteral and enteral nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.935
H-Index - 98
eISSN - 1941-2444
pISSN - 0148-6071
DOI - 10.1002/j.1941-2444.2005.tb04845.x
Subject(s) - hepatectomy , tumor necrosis factor alpha , kupffer cell , medicine , kidney , endocrinology , inflammation , lung , chemistry , surgery , resection
Background : Kupffer cells (KCs) are the resident macrophages of the liver. KCs have an enormous endotoxin eliminating capacity. Endotoxins play an important role in the development of systemic complications after partial hepatectomy by activating KCs. The role of KCs and endotoxins after partial hepatectomy is investigated. Methods : Wistar rats ( n = 16, 250–275 g) were randomly assigned to have 1 mL dichloromethylene‐diphosphonate (CL 2 MDP) or 1 mL NaCl 0.9% IV. Forty‐eight hours later, all rats received a two‐thirds liver resection. Twenty‐four hours later, rats received at random 50 μg/kg endotoxin (LPS) in 1 mL or 1 mL of NaCl 0.9% IV. The rats were killed 4 hours after LPS or SAL infusion. Results : CL 2 MDP infusion resulted in a complete KC elimination. KC‐depleted rats had the lowest mean arterial pressure, the highest heart and ventilatory rate after endotoxemia. All rats were able to maintain pH in normal ranges. The KC‐depleted rats after partial hepatectomy had the lowest CO 2 levels and the highest levels of lactate during endotoxemia. Oxygen levels were similar in all groups. Hepatic, pulmonary, and renal mRNA expression of tumor necrosis factor‐α (TNF‐α) and interleukin‐1β were decreased in KC‐depleted rats. Plasma levels of TNF‐α were significantly decreased in KC‐depleted rats. Furthermore, the highest influx of macrophages and polymorphonuclear cells in the lung and kidney were measured in KC‐depleted rats during endotoxemia. Conclusions : Partial hepatectomy in KC‐depleted rats result in a more pronounced endotoxin‐mediated systemic inflammation and decreased synthesis of cytokines.