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Toward a real liquid biopsy in metastatic breast and prostate cancer: Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap)
Author(s) -
Andree Kiki C.,
Mentink Anouk,
Zeune Leonie L.,
Terstappen Leon W.M.M.,
Stoecklein Nikolas H.,
Neves Rui P.,
Driemel Christiane,
Lampignano Rita,
Yang Liwen,
Neubauer Hans,
Fehm Tanja,
Fischer Johannes C.,
Rossi Elisabetta,
Manicone Mariangela,
Basso Umberto,
Marson Piero,
Zamarchi Rita,
Loriot Yohann,
Lapierre Valerie,
Faugeroux Vincent,
Oulhen Marianne,
Farace Françoise,
Fowler Gemma,
Sousa Fontes Mariane,
Ebbs Berni,
Lambros Maryou,
Crespo Mateus,
Flohr Penny,
Bono Johann S.
Publication year - 2018
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.31752
Subject(s) - circulating tumor cell , medicine , liquid biopsy , prostate cancer , biopsy , breast cancer , oncology , prostate , metastatic breast cancer , cancer , urology , metastasis , pathology
Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL of blood is too small to reliably determine tumor heterogeneity and to be representative as a “liquid biopsy”. In the EU FP7 program CTCTrap, we aimed to validate and optimize the recently introduced Diagnostic LeukApheresis (DLA) to screen liters of blood. Here we present the results obtained from 34 metastatic cancer patients subjected to DLA in the participating institutions. About 7.5 mL blood processed with CellSearch® was used as “gold standard” reference. DLAs were obtained from 22 metastatic prostate and 12 metastatic breast cancer patients at four different institutions without any noticeable side effects. DLA samples were prepared and processed with different analysis techniques. Processing DLA using CellSearch resulted in a 0–32 fold increase in CTC yield compared to processing 7.5 mL blood. Filtration of DLA through 5 μm pores microsieves was accompanied by large CTC losses. Leukocyte depletion of 18 mL followed by CellSearch yielded an increase of the number of CTC but a relative decrease in yield (37%) versus CellSearch DLA. In four out of seven patients with 0 CTC detected in 7.5 mL of blood, CTC were detected in DLA (range 1–4 CTC). The CTC obtained through DLA enables molecular characterization of the tumor. CTC enrichment technologies however still need to be improved to isolate all the CTC present in the DLA.