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Altered expression of specific microRNAs (miRNAs) has been observed in human cervical cancer. However, the biological functions of many of these miRNAs are yet to be discovered. We previously showed that  miR‐944  is significantly more abundant in cervical cancer tissues than their normal counterparts. In this study, we investigated the functions and targets of  miR‐944  in human cervical cancer cells.  MiR‐944  is located in the intron of the tumor protein p63 ( TP63 ) gene, which is frequently overexpressed in cervical carcinomas. Using gain‐ and loss‐of‐function experiments  in vitro , we demonstrate that  miR‐944  promotes cell proliferation, migration and invasion, but has no effect on apoptosis, in human cervical cancer cells. To identify the targets of  miR‐944 , we performed photoactivatable‐ribonucleoside‐enhanced crosslinking and immunoprecipitation followed by deep sequencing. Among the candidate targets, we validated HECW2 (HECT domain ligase W2) and S100PBP (S100P binding protein) as direct targets of  miR‐944  using luciferase reporter assays and western blot analysis. Our findings reveal novel functions and targets of  miR‐944  in human cervical cancer cells, which may provide new insights of its role in cervical carcinogenesis.

Novel functions and targets of miR‐944 in human cervical cancer cells