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Design of experiments using the microcytotoxicity assay
Author(s) -
Brown Joseph P.,
Van Belle Gerald,
Hellström Ingegerd
Publication year - 1976
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910180213
Subject(s) - block (permutation group theory) , variance components , analysis of variance , statistics , variance (accounting) , randomized block design , mathematics , computer science , combinatorics , accounting , business
Abstract Examination of data from the microcytotoxicity assay revealed that the number of surviving target cells varies systematically both between microtest plates and within plates. If the layout of treatments on the plate is not planned carefully, these effects decrease precision and introduce bias. It is recommended that treatments which are to be compared be placed close together on the plate; for example, a plate with 96 wells might be considered as 24 blocks of 4 wells, with the treatments placed within these blocks. Data can be analyzed by means of paired t‐tests or randomized block analysis of variance. These design changes remove the effect of systematic variation and increase the precision of the assay.