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Photocleavage of Peptides and Oligodeoxynucleotides Carrying 2‐Nitrobenzyl Groups
Author(s) -
Ramos Roger,
Manning Brendan,
Aviñó Anna,
Gargallo Raimundo,
Eritja Ramon
Publication year - 2009
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.200800361
Subject(s) - chemistry , intramolecular force , duplex (building) , intermolecular force , dna , aqueous solution , stereochemistry , combinatorial chemistry , molecule , biochemistry , organic chemistry
AbstractPeptides and oligodeoxynucleotides containing photolabile 2‐nitrobenzyl groups as mid‐sequences were prepared. Photocleavage of aqueous solutions of these compounds neared completion within 30 min to a few hours depending on the photolabile group used. A photolabile group was introduced in the loop of an intramolecular oligodeoxynucleotide hairpin. Melting curves of the hairpin with and without the complementary oligodeoxynucleotide showed a preference for the intramolecular hairpin form, but an intermolecular duplex was observed after photolysis. These results open the possibility of using photolabile DNA hairpins for the fabrication of patterned surfaces.