Open AccessNeutrophils alleviate fibrosis in the CCl 4 ‐induced mouse chronic liver injury model
Author(s) -
Saijou Eiko,
Enomoto Yutaka,
Matsuda Michitaka,
YuetYin Kok Cindy,
Akira Shizuo,
Tanaka Minoru,
Miyajima Atsushi
Publication year - 2018
Publication title -
hepatology communications
Language(s) - English
Resource type - Journals
ISSN - 2471-254X
DOI - 10.1002/hep4.1178
Subject(s) - fibrosis , ccl4 , medicine , cxcl1 , liver injury , chemokine , mmp9 , immunology , population , hepatic stellate cell , cancer research , inflammation , endocrinology , downregulation and upregulation , biology , chemistry , biochemistry , carbon tetrachloride , environmental health , organic chemistry , gene
Tribbles pseudokinase 1 ( Trib1 ) is a negative regulator of CCAAT/enhancer binding protein α (C/EBPα) and is known to induce granulopoiesis while suppressing monocyte differentiation. Loss of Trib1 was previously shown to increase the neutrophil population in the spleen but lead to M2‐like macrophage reduction. Because M2 macrophages are anti‐inflammatory and promote tissue repair by producing fibrogenic factors, we investigated liver fibrosis in Trib1 ‐deficient mice. Interestingly, loss of Trib1 suppressed fibrosis in the CCl 4 ‐induced chronic liver injury model. Trib1 knockout increased neutrophils but had a minimal effect on the macrophage population in the liver. Hepatic expressions of neutrophil matrix metalloproteinases ( Mmp ) 8 and Mmp9 were increased, but the production of fibrogenic factors, including transforming growth factor β1, was not affected by loss of Trib1 . These results suggest that neutrophils are responsible for the suppression of fibrosis in Trib1 ‐deficient liver. Consistently, transplantation of Trib1 ‐deficient bone marrow cells into wild‐type mice alleviated CCl 4 ‐induced fibrosis. Furthermore, expression of chemokine (C‐X‐C motif) ligand 1 ( Cxcl1 ) by adeno‐associated viral vector in the normal liver recruited neutrophils and suppressed CCl 4 ‐induced fibrosis; infusion of wild‐type neutrophils in CCl 4 ‐treated mice also ameliorated fibrosis. Using recombinant adeno‐associated virus‐mediated expression of Mmp8 and Mmp9 alleviated liver fibrosis. Finally, neutrophil depletion by infusion of Ly6G antibody significantly enhanced CCl 4 ‐induced fibrosis. Conclusion : While neutrophils are well known to exacerbate acute liver injury, our results demonstrate a beneficial role of neutrophils in chronic liver injury by promoting fibrolysis. ( Hepatology Communications 2018;2:703‐717)