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Msd1/ SSX 2 IP ‐dependent microtubule anchorage ensures spindle orientation and primary cilia formation
Author(s) -
Hori Akiko,
Ikebe Chiho,
Tada Masazumi,
Toda Takashi
Publication year - 2014
Publication title -
embo reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.584
H-Index - 184
eISSN - 1469-3178
pISSN - 1469-221X
DOI - 10.1002/embr.201337929
Subject(s) - library science , microtubule , biology , computer science , microbiology and biotechnology
Abstract Anchoring microtubules to the centrosome is critical for cell geometry and polarity, yet the molecular mechanism remains unknown. Here we show that the conserved human M sd1/ SSX 2I P is required for microtubule anchoring. hM sd1/ SSX 2I P is delivered to the centrosome in a centriolar satellite‐dependent manner and binds the microtubule‐nucleator γ‐tubulin complex. hM sd1/ SSX 2I P depletion leads to disorganised interphase microtubules and misoriented mitotic spindles with reduced length and intensity. Furthermore, hM sd1/ SSX 2I P is essential for ciliogenesis, and during zebrafish embryogenesis, knockdown of its orthologue results in ciliary defects and disturbs left‐right asymmetry. We propose that the M sd1 family comprises conserved microtubule‐anchoring proteins.