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High‐throughput capillary electrophoresis frontal analysis method for the study of drug interactions with human serum albumin at near‐physiological conditions
Author(s) -
MartínezPla Juan J.,
MartínezGómez María A.,
MartínBiosca Yolanda,
Sagrado Salvador,
VillanuevaCamañas Rosa M.,
MedinaHernández Maria J.
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406049
Subject(s) - capillary electrophoresis , human serum albumin , chemistry , chromatography , drug , binding affinities , capillary action , drug discovery , albumin , pharmacology , biochemistry , receptor , biology , materials science , composite material
Abstract The application of the short‐end capillary injection to capillary electrophoresis frontal analysis (CE‐FA) to study the interaction between basic, neutral and acid drugs towards human serum albumin (HSA) at near‐physiological conditions is presented. The compounds selected display a wide range of binding affinities and the results obtained were in good agreement with those reported in the literature. An equation for the estimation of the number of primary binding sites and their corresponding affinity constants is developed isolating the experimentally measured variables in just one axis. The proposed CE‐FA method to screen drug interactions with HSA under physiological conditions is simple, rapid and cost‐effective what may facilitate its implementation in the drug discovery process.