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Use of ethanolamine for sample stacking in capillary electrophoresis
Author(s) -
Louwagie Mathilde,
Rabilloud Thierry,
Garin Jérǒme
Publication year - 1998
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150191414
Subject(s) - capillary electrophoresis , ethanolamine , chromatography , stacking , capillary action , chemistry , resolution (logic) , electrophoresis , phase (matter) , analytical chemistry (journal) , materials science , biochemistry , organic chemistry , computer science , artificial intelligence , composite material
Abstract Capillary zone electrophoresis (CZE) in the presence of ethanolamine was used in a micropreparative mode. Sample volumes up to 1 μL could be loaded onto a 100 μm diameter capillary without loss in resolution. Coupled to narrow‐bore reversed‐phase high‐performance liquid chromatography, ethanolamine‐CZE allowed the collection of sufficient amounts of pure peptidic material to perform amino acid sequence analysis.