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Capillary zone electrophoresis of proteins
Author(s) -
Dolník Vladislav
Publication year - 1997
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150181226
Subject(s) - capillary electrophoresis , chromatography , chemistry , capillary electrochromatography , electrophoresis , capillary electrophoresis–mass spectrometry , capillary action , mass spectrometry , affinity electrophoresis , affinity chromatography , electrospray ionization , biochemistry , materials science , composite material , enzyme
Abstract This review article with 237 references is focused on capillary zone electrophoresis (CZE) of proteins. It includes discussion of modeling electrophoretic migration of proteins, sample pretreatment before the analysis, methods reducing the sorptions of proteins on the capillary wall, and techniques for increasing selectivity by using electrolyte additives including the sieving matrices. Significant progress in detection techniques, namely in laser‐induced fluorescence and mass spectrometry, is emphasized. Modifications of CZE using specific interactions, such as affinity capillary electrophoresis or capillary immunoelectrophoresis are debated as well as combination of CZE with other separation methods such as high performance liquid chromatography (HPLC). A number of practical applications of CZE of proteins are described.