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Development of a Simple and Fast Electrochemical Method to Evaluate Physical Stress in Athletes
Author(s) -
Bezerra da Silva Rodrigo Amorim,
Rodrigues Cunha Rafael,
Coelho da Silva Abílio Tomaz,
Bessa de Oliveira Artur Luis,
Salmen Espindola Foued,
Abarza Munoz Rodrigo Alejandro,
Richter Eduardo Mathias
Publication year - 2011
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201100326
Subject(s) - reagent , saliva , amperometry , chromatography , chemistry , dilution , electrode , electrochemistry , analytical chemistry (journal) , biochemistry , physics , thermodynamics
Abstract This work describes a simple and fast electrochemical method to evaluate the level of physical stress in athletes by monitoring total proteins in saliva. The proposed system is based on FIA with multiple pulse amperometric detection (FIA/MPA) using gold as the working electrode and 0.1 mol L −1 NaOH as the unique reagent. The proposed method is economical, fast (110 injections per hour) and allows the analysis of a small amount of saliva (∼32 µL) with no sample treatment (just dilution). The results obtained by FIA/MPA were compared with the reference spectrophotometric method (Bradford) and a good agreement was found ( r =0.967; p <0.05). In order to investigate a portable system to determinate physical stress, the electrodes were adapted to a batch injection analysis system (BIA) and the current signals of total proteins in saliva were obtained, similar to FIA.