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Direct Bioelectrocatalysis of PQQ‐Dependent Glucose Dehydrogenase
Author(s) -
Ivnitski Dmitri,
Atanassov Plamen,
Apblett Christopher
Publication year - 2007
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200703899
Subject(s) - pyrroloquinoline quinone , carbon nanotube , electron transfer , chemistry , covalent bond , surface modification , electrochemistry , immobilized enzyme , biosensor , chemical engineering , organic chemistry , enzyme , materials science , nanotechnology , electrode , cofactor , biochemistry , engineering
Abstract The direct bioelectrocatalysis was demonstrated for pyrroloquinoline quinone‐dependent glucose dehydrogenase (PQQ‐dependent GDH) covalently attached to single‐walled carbon nanotubes (SWNTs). The homogeneous ink‐like SWNT suspension was used for both creating the SWNT network on the microelectrode carbon surface and for enzyme immobilization. Functionalization of the SWNT surface by forming active ester groups was found to considerably enhance SWNT solubility in water with a range from 0.1 to 1.0 mg/mL. The PQQ‐dependent GDH immobilized on the surface of the SWNTs exhibited fast heterogeneous electron transfer with a rate constant of 3.6 s −1 . Moreover, the immobilized PQQ‐dependent GDH retained its enzymatic activity for glucose oxidation. A fusion of PQQ‐dependent GDH with SWNTs has a great potential for the development of low‐cost and reagentless glucose sensors and biofuel cells.