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Adsorptive stripping square‐wave voltammetric behavior of the enzyme adenosine deaminase
Author(s) -
Flores Juana Rodríguez,
Alvarez José María Fernández
Publication year - 1992
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140040313
Subject(s) - adenosine deaminase , square wave , stripping (fiber) , chemistry , adsorption , analytical chemistry (journal) , aqueous solution , pulse (music) , adenosine , square (algebra) , chromatography , materials science , mathematics , physics , voltage , biochemistry , quantum mechanics , geometry , composite material
Abstract The adsorptive stripping voltammetric behavior of adenosine deaminase has been studied by both square‐wave and differential pulse modes, leading to analytical methodologies for its determination in aqueous samples. The application of a square‐wave mode for the stripping of adsorbed adenosine deaminase has proved to be much more sensitive, yielding signals 50 times larger than those obtained by applying a differential pulse scan. The precision obtained for both voltammetric methods, at a concentration level as low as 8 ppb of the enzyme, was shown to be 2.47% (r.s.d., n = 7) and 2.28% (r.s.d., n = 7), respectively.