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Protein‐Templated Dynamic Combinatorial Chemistry: Brief Overview and Experimental Protocol
Author(s) -
Hartman Alwin M.,
Gierse Robin M.,
Hirsch Anna K. H.
Publication year - 2019
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/ejoc.201900327
Subject(s) - chemistry , protocol (science) , combinatorial chemistry , nanotechnology , computational biology , protein chemistry , biochemical engineering , biochemistry , medicine , materials science , alternative medicine , pathology , engineering , biology
Dynamic combinatorial chemistry (DCC) is a powerful tool to identify bioactive compounds. This efficient technique allows the target to select its own binders and circumvents the need for synthesis and biochemical evaluation of all individual derivatives. An ever‐increasing number of publications report the use of DCC on biologically relevant target proteins. This minireview complements previous reviews by focusing on the experimental protocol and giving detailed examples of essential steps and factors that need to be considered, such as protein stability, buffer composition and cosolvents.