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Site‐Selective Ser‐Hydrolase Labelling with a Luminescent Organometallic NCN–Platinum Complex
Author(s) -
Wieczorek Birgit,
Lemcke Bart,
Dijkstra Harm P.,
Egmond Maarten R.,
Klein Gebbink Robertus J. M.,
van Koten Gerard
Publication year - 2010
Publication title -
european journal of inorganic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.667
H-Index - 136
eISSN - 1099-0682
pISSN - 1434-1948
DOI - 10.1002/ejic.200900980
Subject(s) - chemistry , platinum , luminescence , phosphonate , serine hydrolase , serine , hydrolase , organometallic chemistry , labelling , stereochemistry , photochemistry , enzyme , organic chemistry , biochemistry , catalysis , physics , optoelectronics
The synthesis, spectroscopic properties and protein‐binding studies of a novel luminescent organometallic NCN–platinum complex are described. The luminescent organometallic complex was linked to a serine hydrolase reactive phosphonate group by means of click chemistry, and was exploited in serine hydrolase specific‐binding studies using gel electrophoresis. The NCN–platinum protein label was found to be a robust dye with absorbance and emission maxima between 374–380 and 482–493 nm, respectively, and quantum yields between 0.04 and 0.15.