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OMIP‐022: Comprehensive assessment of antigen‐specific human T‐cell functionality and memory
Author(s) -
Graves Andrew J.,
Padilla Marcelino G.,
Hokey David A.
Publication year - 2014
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.22478
Subject(s) - immunology , antigen , cytokine , cd154 , immune system , t cell , cd8 , biology , medicine , cd40 , cytotoxic t cell , in vitro , biochemistry
THIS flow cytometry antibody panel was developed and optimized for the characterization of CD41 and CD81 T-cell memory and functional responses in adult and infant cryopreserved peripheral blood mononuclear cells (PBMC) stimulated with peptide pools to various antigens of interest (Table 1). The panel has been used to evaluate Mycobacterium tuberculosis (TB) antigen-specific responses in clinical trial specimens, and is currently undergoing assay qualification.