English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

The molecular profile of neurotrophic tyrosine kinase receptor ( NTRK ) gene fusions in lung adenocarcinoma (LUAD) is not fully understood. Next‐generation sequencing (NGS) and pan‐tyrosine kinase receptor (TRK) immunohistochemistry (IHC) are powerful tools for  NTRK  fusion detection. In this study, a total of 4,619 LUAD formalin‐fixed, paraffin‐embedded tissues were collected from patients who underwent biopsy or resection at the Shanghai Chest Hospital during 2017–2019. All specimens were screened for  NTRK1  rearrangements using DNA‐based NGS. Thereafter, the cases with  NTRK1  rearrangements and cases negative for common driver mutations were analyzed for  NTRK1 / 2 / 3  fusions using total nucleic acid (TNA)‐based NGS and pan‐TRK IHC. Overall, four  NTRK1/2  fusion events were identified, representing 0.087% of the original sample set. At the DNA level, seven  NTRK1  rearrangements were identified, while only two  TPM3‐NTRK1  fusions were confirmed on TNA‐based NGS as functional. In addition, two  NTRK2  fusions ( SQSTM1‐NTRK2  and  KIF5B‐NTRK2 ) were identified by TNA‐based NGS in 350 ‘pan‐negative’ cases. Two patients harboring  NTRK1 / 2  fusions were diagnosed with invasive adenocarcinoma, while the other two were diagnosed with adenocarcinoma  in situ  and minimally invasive adenocarcinoma. All four samples with  NTRK  fusions were positive for the expression of pan‐TRK. The two samples with  NTRK2  fusions showed cytoplasmic staining alone, while the other two samples with  NTRK1  fusions exhibited both cytoplasmic and membranous staining. In summary, functional  NTRK  fusions are found in early‐stage LUAD; however, they are extremely rare. According to this study's results, they are independent oncogenic drivers, mutually exclusive with other driver mutations. We demonstrated that NTRK rearrangement analysis using a DNA‐based approach should be verified with an RNA‐based assay.

Identification of NTRK gene fusions in lung adenocarcinomas in the Chinese population