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Detecting mutations in single cells from cancer specimens is now a major area of translational research. In a recent article in this journal, Khalique  et al  validated an immunohistochemistry assay for ARID1A that reliably identifies loss of function mutations in single cells in tissue sections. This work exemplifies best practice for developing and orthogonally validating immunohistochemical assays to provide clearly interpretable mutational results with spatial context.

You won't believe this old test … that does cheap single‐cell mutation detection