Open AccessYou won't believe this old test … that does cheap single‐cell mutation detection
Author(s) -
Köbel Martin,
Anglesio Michael S.,
Brenton James D.
Publication year - 2018
Publication title -
the journal of pathology: clinical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.849
H-Index - 21
ISSN - 2056-4538
DOI - 10.1002/cjp2.108
Subject(s) - immunohistochemistry , context (archaeology) , mutation , loss function , function (biology) , biology , computational biology , cancer research , genetics , microbiology and biotechnology , gene , phenotype , immunology , paleontology
Detecting mutations in single cells from cancer specimens is now a major area of translational research. In a recent article in this journal, Khalique et al validated an immunohistochemistry assay for ARID1A that reliably identifies loss of function mutations in single cells in tissue sections. This work exemplifies best practice for developing and orthogonally validating immunohistochemical assays to provide clearly interpretable mutational results with spatial context.