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A Novel Visible Range FRET Probe for Monitoring Acid Sphingomyelinase Activity in Living Cells
Author(s) -
Kappe Christian,
Mohamed Zainelabdeen H.,
Naser Eyad,
Carpinteiro Alexander,
Arenz Christoph
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202000133
Subject(s) - förster resonance energy transfer , acid sphingomyelinase , sphingomyelin , range (aeronautics) , biophysics , chemistry , nanotechnology , materials science , biology , optics , biochemistry , physics , fluorescence , membrane , composite material
Activity of acid sphingomyelinase has been implicated in a number of diseases like acute lung injury, sepsis or metastasis of melanoma cells. Here, we present a sphingomyelinase FRET probe based on FAM/BODIPY dyes for real‐time monitoring of acid sphingomyelinase. The probe gives rise to a tremendous increase in fluorescence of the fluorescein FRET donor upon cleavage and we show that this is, to a significant part, due to cleavage‐associated phase transition, suggesting a more systematic consideration of such effects for future probe development. The probe allows for the first time to monitor relative sphingomyelinase activities of intact living cells by flow cytometry.