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Molecular Encapsulation Inside Microtubules Based on Tau‐Derived Peptides
Author(s) -
Inaba Hiroshi,
Yamamoto Takahisa,
Kabir Arif Md. Rashedul,
Kakugo Akira,
Sada Kazuki,
Matsuura Kazunori
Publication year - 2018
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201802617
Subject(s) - microtubule , tubulin , cytoskeleton , biophysics , chemistry , nanomaterials , peptide , nanotechnology , nanocapsules , nanoparticle , materials science , microbiology and biotechnology , biology , biochemistry , cell
Microtubules are cytoskeletal filaments that serve as attractive scaffolds for developing nanomaterials and nanodevices because of their unique structural properties. The functionalization of the outer surface of microtubules has been established for this purpose. However, no attempts have been made to encapsulate molecules inside microtubules with 15 nm inner diameter. The encapsulation of various molecular cargos inside microtubules constitutes a new concept for nanodevice and nanocarrier applications of microtubules. Here, we developed peptide motifs for binding to the inner surface of microtubules, based on a repeat domain of the microtubule‐associated protein Tau. One of the four Tau‐derived peptides, 2 N , binds to a taxol binding pocket of β‐tubulin located inside microtubules by preincubation with tubulin dimer and subsequent polymerization of the peptide‐tubulin complex. By conjugation of 2 N to gold nanoparticles, encapsulation of gold nanoparticles inside microtubules was achieved. The methodology for molecular encapsulation inside microtubules by the Tau‐derived peptide is expected to advance the development of microtubule‐based nanomaterials and nanodevices.