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Luminescent Amphiphilic Aminoglycoside Probes to Study Transfection
Author(s) -
Zimmermann Alexander,
Jaber Qais Z.,
Koch Johannes,
Riebe Steffen,
Vallet Cecilia,
Loza Kateryna,
Hayduk Matthias,
Steinbuch Kfir B.,
Knauer Shirley K.,
Fridman Micha,
Voskuhl Jens
Publication year - 2021
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.202000725
Subject(s) - transfection , lipofectamine , amphiphile , chemistry , luminescence , biophysics , cationic polymerization , materials science , biochemistry , biology , organic chemistry , recombinant dna , optoelectronics , vector (molecular biology) , copolymer , gene , polymer
We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation‐induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding results in an emission “on” signal due to restriction of intramolecular motion of the luminophore core. The luminescent cationic amphiphiles effectively transferred plasmid DNA into mammalian cells (HeLa, HEK 293T), as proven by expression of a red fluorescent protein marker. The morphologies of the aggregates were investigated by microscopy as well as ζ‐potential and dynamic light‐scattering measurements. The transfection efficiencies using luminescent cationic amphiphiles were similar to that of the gold‐standard transfection reagent Lipofectamine® 2000.