Premium
A Photoenzymatic NADH Regeneration System
Author(s) -
Höfler Georg T.,
FernándezFueyo Elena,
Pesic Milja,
Younes Sabry H.,
Choi EunGyu,
Kim Yong H.,
Urlacher Vlada B.,
Arends Isabel W. C. E.,
Hollmann Frank
Publication year - 2018
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201800530
Subject(s) - nad+ kinase , stereospecificity , alcohol dehydrogenase , regeneration (biology) , chemistry , reductase , enzyme , dehydrogenase , nadh dehydrogenase , biochemistry , redox , reduction (mathematics) , biocatalysis , catalysis , cofactor , combinatorial chemistry , stereochemistry , biology , reaction mechanism , organic chemistry , microbiology and biotechnology , geometry , mathematics , protein subunit , gene
A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD + into the enzyme‐active 1,4‐NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.