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Rapid and sensitive determination of ephedrine and pseudoephedrine by micellar electrokinetic chromatography with on‐line regenerating covalent coating
Author(s) -
Jianping Xie,
Jiyou Zhang,
Jiaqin Liu,
Jianniao Tian,
Xingguo Chen,
Zhide Hu
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.407
Subject(s) - chemistry , pseudoephedrine , ephedrine , chromatography , micellar electrokinetic chromatography , electrokinetic phenomena , coating , covalent bond , electrophoresis , organic chemistry , pharmacology , medicine
Abstract A rapid, sensitive and reproducible micellar electrokinetic chromatographic method using hexamethyldisilazane as on‐line regenerating covalent coating was developed for the quantication of ephedrine (E) and pseudoephedrine (PE). E and PE were derivatized with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazol for laser‐induced uorescence detection. The on‐line regenerating covalent coating formed a combinative double coating with the subsequently produced dynamic SDS coating. The total coating can be easily removed and conveniently regenerated on‐line. The simple coating procedure was described. By a series of optimization, a running buffer of 20 m m Na 2 B 4 O 7 + 16 m m SDS was applied for the separation of the derivatives. Linear relationships for E and PE were obtained in the range of 0.044–6.60 µg mL −1 (correlation coefcients: 0.9975 for E, 0.9981 for PE), and the detection limits for E and PE were 1.71 and 0.67 ng mL −1 , respectively. The separation speed, the reproducibility and the sensitivity were much improved over those of other capillary electrophoresis methods more recently reported. The method was applied to the analysis of the two alkaloids in traditional herbal preparations with recoveries in the range 92.8–104.8%. Copyright © 2004 John Wiley & Sons, Ltd.