Premium
1 H‐NMR‐based Metabolomics Studies of the Toxicity of Mesoporous Carbon Nanoparticles in Zebrafish ( Danio rerio )
Author(s) -
Raja Ganesan,
Kim Siwon,
Yoon Dahye,
Yoon Changshin,
Kim Suhkmann
Publication year - 2017
Publication title -
bulletin of the korean chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.237
H-Index - 59
ISSN - 1229-5949
DOI - 10.1002/bkcs.11080
Subject(s) - danio , metabolomics , zebrafish , chemistry , citric acid cycle , tricarboxylic acid , metabolome , metabolite , biochemistry , metabolism , chromatography , gene
Mesoporous carbon nanoparticles ( MCNs ) have been applied in a variety of drug/gene carriers. In addition to their potential benefits, many studies of their potential toxicity have been reported, showing the limitations of metabolic contextualization. In this study, we conducted 1 H ‐nuclear magnetic resonance ( NMR ) profiling combined with statistical methods such as orthogonal partial least squares discriminant analysis and Pearson correlation analysis to assess metabolic alterations in the whole body of zebrafish ( Danio rerio ) in the presence of various concentrations of MCNs . The MCN exposure influenced numerous metabolites in energy metabolism ( e . g ., metabolites involved in glycolysis and tricarboxylic acid cycle) and disturbed the balance of neurotransmitters and osmoregulators. Our findings demonstrate the potential applicability of using a metabolomics approach to determine underlying metabolic disturbances caused by MCNs .