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Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors
Author(s) -
Rodolfi Liliana,
Biondi Natascia,
Guccione Alessia,
Bassi Niccolò,
D'Ottavio Massimo,
Arganaraz Gimena,
Tredici Mario R.
Publication year - 2017
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.26353
Subject(s) - phaeodactylum tricornutum , eicosapentaenoic acid , productivity , polyunsaturated fatty acid , biomass (ecology) , food science , nitrogen , hectare , nutrient , biology , diatom , botany , fatty acid , chemistry , environmental chemistry , agronomy , biochemistry , ecology , organic chemistry , macroeconomics , economics , agriculture
Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen availability affect productivity, oil yield, and fatty acid (FA) composition of P. tricornutum UTEX 640. After preliminary laboratory trials, outdoor experiments were carried out in 40‐L GWP® reactors under different nitrogen regimes in batch. Nitrogen replete cultures achieved the highest productivity of biomass (about 18 g m −2 d −1 ) and EPA (about 0.35 g m −2 d −1 ), whereas nitrogen‐starved cultures achieved the highest FA productivity (about 2.6 g m −2 d −1 ). The annual potential yield of P. tricornutum grown outdoors in GWP® reactors is 730 kg of EPA per hectare under nutrient‐replete conditions and 5,800 kg of FA per hectare under nitrogen starvation. Biotechnol. Bioeng. 2017;114: 2204–2210. © 2017 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.