Premium
One‐ and two‐photon induced fluorescence of Pacific Blue‐labeled human serum albumin deposited on different core size silver colloids
Author(s) -
Lukomska Joanna,
Gryczynski Ignacy,
Malicka Joanna,
Makowiec Slawomir,
Lakowicz Joseph R.,
Gryczynski Zygmunt
Publication year - 2006
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20407
Subject(s) - fluorescence , chemistry , excitation , colloid , two photon excitation microscopy , brightness , photon , analytical chemistry (journal) , photochemistry , nanotechnology , optics , materials science , chromatography , physics , quantum mechanics
Abstract We studied one‐ and two‐photon induced fluorescence of Pacific Blue (PB)‐labeled human serum albumin (HSA) in the presence of different size silver colloids. The PB fluorescence emission intensity was observed with small (30–40 nm) and large (about 120 nm) colloids and compared with PB emission in absence of colloids. For the system with a small core size colloids we did not detect any fluorescence enhancement with one‐photon excitation and the enhancement observed with two‐photon excitation was about 2.5‐fold. In contrast, for large silver colloids we observed about a 2‐fold increase in PB fluorescence brightness for one‐photon excitation, and the enhancement with two‐photon excitation excided 13‐folds. Much stronger increases in brightness observed with two‐photon excitation, compared to one‐photon excitation, indicate a dominant role of enhanced local field in fluorescence enhancement on silver colloids in solutions. © 2005 Wiley Periodicals, Inc. Biopolymers 81: 249–255, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com