Determination of norfloxacin using a terbium‐sensitized electrogenerated chemiluminescence method

A simple electrogenerated chemiluminescence (ECL) analysis method for the determination of norfloxacin (NFLX) is reported. It is based on ECL produced by Na 2 SO 3 , which is sensitized by the Tb–NFLX complex. The relative ECL intensity of the Tb 3+ –NFLX–Na 2 SO 3 system is proportional to the amount of NFLX. The optimized experimental conditions were investigated. The linear range and detection limit for NFLX were 1.0 × 10 −10 –8.0 × 10 −7 mol/L and 2.8 × 10 −11 mol/L, respectively. This method was successfully applied to the determination of NFLX in a capsule. NFLX in urine can be directly detected without pretreatment or separation. Copyright © 2005 John Wiley & Sons, Ltd.