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Determination of norfloxacin using a terbium‐sensitized electrogenerated chemiluminescence method
Author(s) -
Chen ShiLv,
Liu Yu,
Zhao HuiChun,
Jin LinPei,
Zhang ZhongLun,
Zheng YanZhen
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.876
Subject(s) - norfloxacin , chemiluminescence , terbium , detection limit , chemistry , linear range , chromatography , analytical chemistry (journal) , ciprofloxacin , antibiotics , organic chemistry , ion , biochemistry
Abstract A simple electrogenerated chemiluminescence (ECL) analysis method for the determination of norfloxacin (NFLX) is reported. It is based on ECL produced by Na 2 SO 3 , which is sensitized by the Tb–NFLX complex. The relative ECL intensity of the Tb 3+ –NFLX–Na 2 SO 3 system is proportional to the amount of NFLX. The optimized experimental conditions were investigated. The linear range and detection limit for NFLX were 1.0 × 10 −10 –8.0 × 10 −7 mol/L and 2.8 × 10 −11 mol/L, respectively. This method was successfully applied to the determination of NFLX in a capsule. NFLX in urine can be directly detected without pretreatment or separation. Copyright © 2005 John Wiley & Sons, Ltd.