Premium
Activation of the Catalytic Activity of Thrombin for Fibrin Formation by Ultrasound
Author(s) -
Zhao Pengkun,
Huo Shuaidong,
Fan Jilin,
Chen Junlin,
Kiessling Fabian,
Boersma Arnold J.,
Göstl Robert,
Herrmann Andreas
Publication year - 2021
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.202105404
Subject(s) - thrombin , fibrin , aptamer , chemistry , biophysics , enzyme , hemostasis , colloidal gold , nanoparticle , biochemistry , nanotechnology , materials science , microbiology and biotechnology , platelet , biology , immunology , medicine , surgery
The regulation of enzyme activity is a method to control biological function. We report two systems enabling the ultrasound‐induced activation of thrombin, which is vital for secondary hemostasis. First, we designed polyaptamers, which can specifically bind to thrombin, inhibiting its catalytic activity. With ultrasound generating inertial cavitation and therapeutic medical focused ultrasound, the interactions between polyaptamer and enzyme are cleaved, restoring the activity to catalyze the conversion of fibrinogen into fibrin. Second, we used split aptamers conjugated to the surface of gold nanoparticles (AuNPs). In the presence of thrombin, these assemble into an aptamer tertiary structure, induce AuNP aggregation, and deactivate the enzyme. By ultrasonication, the AuNP aggregates reversibly disassemble releasing and activating the enzyme. We envision that this approach will be a blueprint to control the function of other proteins by mechanical stimuli in the sonogenetics field.