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NMR Characterization of Surface Receptor Protein Interactions in Live Cells Using Methylcellulose Hydrogels
Author(s) -
Mateos Borja,
SealeyCardona Marco,
Balazs Katja,
Konrat Judith,
Staffler Guenther,
Konrat Robert
Publication year - 2020
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201913585
Subject(s) - internalization , extracellular , self healing hydrogels , receptor , chemistry , biophysics , transmembrane protein , cell membrane , microbiology and biotechnology , cell surface receptor , cell , drug discovery , membrane protein , force spectroscopy , g protein coupled receptor , membrane , nanotechnology , biochemistry , biology , materials science , atomic force microscopy , organic chemistry
Abstract Interactions of transmembrane receptors with their extracellular ligands are essential for cellular communication and signaling and are therefore a major focus in drug discovery programs. The transition from in vitro to live cell interaction studies, however, is typically a bottleneck in many drug discovery projects due to the challenge of obtaining atomic‐resolution information under near‐physiological conditions. Although NMR spectroscopy is ideally suited to overcome this limitation, several experimental impairments are still present. Herein, we propose the use of methylcellulose hydrogels to study extracellular proteins and their interactions with plasma membrane receptors. This approach reduces cell sedimentation, prevents the internalization of membrane receptors, and increases cell survival, while retaining the free tumbling of extracellular proteins.