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Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal Pt II Metallacycle
Author(s) -
Domarco Olaya,
Kieler Claudia,
Pirker Christine,
Dinhof Carina,
Englinger Bernhard,
Reisecker Johannes M.,
Timelthaler Gerald,
García Marcos D.,
Peinador Carlos,
Keppler Bernhard K.,
Berger Walter,
Terenzi Alessio
Publication year - 2019
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201900934
Subject(s) - metallacycle , chromatin , photobleaching , fluorescence , supramolecular chemistry , chemistry , duplex (building) , dna , g quadruplex , biophysics , crystallography , biology , biochemistry , physics , crystal structure , x ray crystallography , diffraction , optics , quantum mechanics
Metal‐driven self‐assembly afforded a multitude of fascinating supramolecular coordination complexes (SCCs) with applications as catalysts, host–guest, and stimuli‐responsive systems. However, the interest in the biological applications of SCCs is only starting to emerge and thorough characterization of their behavior in biological milieus is still lacking. Herein, we report on the synthesis and detailed in‐cell tracking of a Pt 2 L 2 metallacycle. We show that our hexagonal supramolecule accumulates in cancer cell nuclei, exerting a distinctive blue fluorescence staining of chromatin resistant to UV photobleaching selectively in nucleolar G4‐rich regions. SCC co‐localizes with epitopes of the quadruplex‐specific antibody BG4 and replaces other well‐known G4 stabilizers. Moreover, the photophysical changes accompanying the metallacycle binding to G4s in solution (fluorescence quenching, absorption enhancement) also take place intracellularly, allowing its subcellular interaction tracking.