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Covalent Lectin Inhibition and Application in Bacterial Biofilm Imaging
Author(s) -
Wagner Stefanie,
Hauck Dirk,
Hoffmann Michael,
Sommer Roman,
Joachim Ines,
Müller Rolf,
Imberty Anne,
Varrot Annabelle,
Titz Alexander
Publication year - 2017
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201709368
Subject(s) - biofilm , pseudomonas aeruginosa , virulence , lectin , microbiology and biotechnology , pathogen , chemistry , virulence factor , bacteria , pathogenic bacteria , human pathogen , covalent bond , biochemistry , biology , gene , genetics , organic chemistry
Biofilm formation by pathogenic bacteria is a hallmark of chronic infections. In many cases, lectins play key roles in establishing biofilms. The pathogen Pseudomonas aeruginosa often exhibiting various drug resistances employs its lectins LecA and LecB as virulence factors and biofilm building blocks. Therefore, inhibition of the function of these proteins is thought to have potential in developing “pathoblockers” preventing biofilm formation and virulence. A covalent lectin inhibitor specific to a carbohydrate binding site is described for the first time. Its application in the LecA‐specific in vitro imaging of biofilms formed by P. aeruginosa is also reported.