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Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells
Author(s) -
Butkevich Alexey N.,
Mitronova Gyuzel Yu.,
Sidenstein Sven C.,
Klocke Jessica L.,
Kamin Dirk,
Meineke Dirk N. H.,
D'Este Elisa,
Kraemer PhilipTobias,
Danzl Johann G.,
Belov Vladimir N.,
Hell Stefan W.
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201511018
Subject(s) - sted microscopy , fluorescence , microscopy , fluorescence microscope , super resolution microscopy , resolution (logic) , absorption (acoustics) , chemistry , biophysics , stimulated emission , materials science , optics , laser , biology , physics , artificial intelligence , computer science
A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ =500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μ m solutions of the dye–ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure–property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one‐ and two‐color images of living cells with an optical resolution of 40–60 nm.