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iBodies: Modular Synthetic Antibody Mimetics Based on Hydrophilic Polymers Decorated with Functional Moieties
Author(s) -
Šácha Pavel,
Knedlík Tomáš,
Schimer Jiří,
Tykvart Jan,
Parolek Jan,
Navrátil Václav,
Dvořáková Petra,
Sedlák František,
Ulbrich Karel,
Strohalm Jiří,
Majer Pavel,
Šubr Vladimír,
Konvalinka Jan
Publication year - 2016
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201508642
Subject(s) - conjugate , combinatorial chemistry , flow cytometry , chemistry , ligand (biochemistry) , polymer , immunoprecipitation , antibody , target protein , modular design , nanotechnology , biochemistry , materials science , computer science , microbiology and biotechnology , biology , organic chemistry , receptor , mathematical analysis , operating system , mathematics , gene , immunology
Antibodies are indispensable tools for biomedicine and anticancer therapy. Nevertheless, their use is compromised by high production costs, limited stability, and difficulty of chemical modification. The design and preparation of synthetic polymer conjugates capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, immunocytochemistry, and immunoprecipitation is reported. The conjugates, named “iBodies”, consist of an HPMA copolymer decorated with low‐molecular‐weight compounds that function as targeting ligands, affinity anchors, and imaging probes. We prepared specific conjugates targeting several proteins with known ligands and used these iBodies for enzyme inhibition, protein isolation, immobilization, quantification, and live‐cell imaging. Our data indicate that this highly modular and versatile polymer system can be used to produce inexpensive and stable antibody substitutes directed toward virtually any protein of interest with a known ligand.