Nonenzymatic Polymerization of Ubiquitin: Single‐Step Synthesis and Isolation of Discrete Ubiquitin Oligomers

Linked : A method based on thiol‐ene chemistry enables the synthesis and purification of ubiquitin oligomers with ≥4 units. This approach, which employs free‐radical polymerization, can be applied towards the synthesis of homogeneous Lys6‐linked ubiquitin oligomers currently inaccessible by enzymatic methods. By using these chains, one can study their roles in the ubiquitin proteasome system and the DNA damage response pathway.