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Nonenzymatic Polymerization of Ubiquitin: Single‐Step Synthesis and Isolation of Discrete Ubiquitin Oligomers
Author(s) -
Trang Vivian H.,
Valkevich Ellen M.,
Minami Shoko,
Chen YiChen,
Ge Ying,
Strieter Eric R.
Publication year - 2012
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.201207171
Subject(s) - ubiquitin , polymerization , homogeneous , chemistry , proteasome , dna , biochemistry , computational biology , computer science , combinatorial chemistry , biology , organic chemistry , polymer , mathematics , combinatorics , gene
Linked : A method based on thiol‐ene chemistry enables the synthesis and purification of ubiquitin oligomers with ≥4 units. This approach, which employs free‐radical polymerization, can be applied towards the synthesis of homogeneous Lys6‐linked ubiquitin oligomers currently inaccessible by enzymatic methods. By using these chains, one can study their roles in the ubiquitin proteasome system and the DNA damage response pathway.