Open AccessMicroarray microRNA profiling of urinary exosomes in a 5XFAD mouse model of Alzheimer’s disease
Author(s) -
Song Zhiqi,
Qu Yajin,
Xu Yanfeng,
Zhang Ling,
Zhou Li,
Han Yunlin,
Zhao Wenjie,
Yu Pin,
Zhang Yu,
Li Xianglei,
Qin Chuan
Publication year - 2021
Publication title -
animal models and experimental medicine
Language(s) - English
Resource type - Journals
ISSN - 2576-2095
DOI - 10.1002/ame2.12175
Subject(s) - microrna , microvesicles , pathogenesis , microarray , microarray analysis techniques , disease , gene expression profiling , biology , downregulation and upregulation , digital polymerase chain reaction , exosome , transcriptome , cancer research , pathology , bioinformatics , gene expression , medicine , gene , immunology , genetics , polymerase chain reaction
Background Alzheimer's disease (AD) is an incurable and irreversible neurodegenerative disease, without a clear pathogenesis. Therefore, identification of candidates before amyloid‐β plaque (Aβ) deposition proceeds is of major significance for earlier intervention in AD. Methods To explore the potential noninvasive earlier biomarkers of AD in a 5XFAD mouse model, microRNAs (miRNAs) from urinary exosomes in 1‐month‐old pre‐Aβ accumulation 5XFAD mice models and their littermate controls were profiled by microarray analysis. The differentially expressed miRNAs were further analyzed via droplet digital PCR (ddPCR). Results Microarray analysis demonstrated that 48 differentially expressed miRNAs (18 upregulated and 30 downregulated), of which six miRNAs – miR‐196b‐5p, miR‐339‐3p, miR‐34a‐5p, miR‐376b‐3p, miR‐677‐5p, and miR‐721 – were predicted to display gene targets and important signaling pathways closely associated with AD pathogenesis and verified by ddPCR. Conclusions Urinary exosomal miRNAs showing differences in expression prior to Aβ‐plaque deposition were identified. These exosomal miRNAs represent potential noninvasive biomarkers that may be used to prevent AD in clinical applications.