Open AccessIsolation and characterization of Bordetella pseudohinzii in mice in China
Author(s) -
Ma Lei,
Huang Shuwu,
Luo Yinzhu,
Min Fangui,
He Lifang,
Chen Meiling,
Pan Jinchun,
Zhang Yu,
Wang Jing
Publication year - 2019
Publication title -
animal models and experimental medicine
Language(s) - English
Resource type - Journals
ISSN - 2576-2095
DOI - 10.1002/ame2.12075
Subject(s) - biology , feces , isolation (microbiology) , microbiology and biotechnology , 16s ribosomal rna , pathogen , virology , phylogenetic tree , specific pathogen free , genome , clade , bacteria , gene , virus , genetics
We report on the first detection and isolation of B. pseudohinzii ( Bordetella pseudohinzii ) in laboratory mice in China. Forty‐one B. pseudohinzii strains were isolated from 3094 mice in 33 different laboratory animal facilities in southern China. The isolates were identified through culture and genome sequenceing. Phylogenetic analysis based on the sequences of 16S rRNA and OmpA genes demonstrated that these strains were on the same clade as other B. pseudohinzii strains isolated from mice. Experimental infected mice presented an asymptomatic infection. B. pseudohinzii replicated in both the respiratory tract and the digestive tract. Most importantly B. pseudohinzii shed via feces and infected a group of sentinel mice in a separate cage via cage padding contaminated with B. pseudohinzii ‐positive feces, indicating that B. pseudohinzii could transmit efficiently among mice and contaminate environmental facilities. Our study highlights the importance of routine monitoring of the pathogen in laboratory mice and provides vital insights into the transmission of Brodetellae in rodents and human.