Open AccessCell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation
Author(s) -
Sório Ana Luisa Riul,
VargasSanchez Paula Katherine,
Fernandes Roger Rodrigo,
Pitol Dimitrius Leonardo,
de Sousa Luiz Gustavo,
Bianchini Adriano Luiz Balthazar,
de Melo Geraldo Batista,
Siessere Selma,
BombonatoPrado Karina Fittipaldi
Publication year - 2019
Publication title -
animal models and experimental medicine
Language(s) - English
Resource type - Journals
ISSN - 2576-2095
DOI - 10.1002/ame2.12073
Subject(s) - calvaria , irradiation , bone formation , chemistry , biophysics , medicine , biology , endocrinology , biochemistry , in vitro , physics , nuclear physics
Background The purpose of the study was to analyze the effect of cell therapy on the repair process in calvaria defects in rats subjected to irradiation. Methods Bone marrow mesenchymal cells were characterized for osteoblastic phenotype. Calvariae of male Wistar rats were irradiated (20 Gy) and, after 4 weeks, osteoblastic cells were placed in surgically created defects in irradiated (IRC) and control animals (CC), paired with untreated irradiated (IR) and control (C) animals. After 30 days, histological and microtomographic evaluation was performed to establish significant ( P < 0.05) differences among the groups. Results Higher alkaline phosphatase detection and activity, along with an increase in mineralized nodules, in the IRC, C and CC groups compared to the IR group, confirmed an osteoblastic phenotype. Histology showed impaired bone neoformation following irradiation, affecting bone marrow composition. Cell therapy in the IRC group improved bone neoformation compared to the IR group. Microtomography revealed increased bone volume, bone surface and trabecular number in IRC group compared to the IR group. Conclusion Cell therapy may improve bone neoformation in defects created after irradiation.