Open AccessLgr5 + intestinal stem cell sorting and organoid culture
Author(s) -
Liu Ruixue,
Li Haifeng,
Cai Juan,
Wei Qiang,
Han Xiaonan
Publication year - 2019
Publication title -
animal models and experimental medicine
Language(s) - English
Resource type - Journals
ISSN - 2576-2095
DOI - 10.1002/ame2.12057
Subject(s) - lgr5 , organoid , stem cell , sorting , crypt , biology , microbiology and biotechnology , computer science , cancer stem cell , programming language , endocrinology
Intestinal epithelial stem cells (IESCs) are one of the most rapidly self‐renewing and proliferating adult stem cells. The IESCs reside at the bottom of intestinal and colonic crypts, giving rise to all intestinal epithelial lineages and maintaining intestinal epithelial replenishment. The technique of three‐dimensional culture based upon intestinal stem cell biology has been recently developed to study gastrointestinal development and disease pathogenesis. Here, we summarize the techniques used to isolate Lgr5‐positive IESCs to form the enteroids from intestine or colonoids from colon, and present the means to examine these organoid functions. This study will provide a simple and practical way for producing intestinal tissues in the laboratory.