Open AccessComparative medical characteristics of ZDF ‐T2 DM rats during the course of development to late stage disease
Author(s) -
Pang YunLi,
Hu JingWen,
Liu GuangLong,
Lu ShuaiYao
Publication year - 2018
Publication title -
animal models and experimental medicine
Language(s) - English
Resource type - Journals
ISSN - 2576-2095
DOI - 10.1002/ame2.12030
Subject(s) - medicine , endocrinology , insulin , insulin receptor , insulin resistance , leptin receptor , adiponectin , irs1 , type 2 diabetes , insulin receptor substrate , diabetes mellitus , leptin , type 2 diabetes mellitus , irs2 , blood sugar , glycogen , obesity
Background There are few reports on the comparative medical characteristics of type 2 diabetes models in late stage. An analysis of comparative medical characteristics of Zucker diabetic fatty type 2 diabetes mellitus ( ZDF ‐T2 DM ) rats during the course of development to late stage disease was performed. Methods In this study, ZDF rats were fed with high‐sugar and high‐fat diets to raise the fasting blood glucose, and develop of type 2 diabetes. At the late stage of T2 DM , the preliminary comparative medical characteristics of the T2 DM model were analyzed through the detection of clinical indicators, histopathology, related cytokine levels, and insulin‐related signaling molecule expression levels. Results In the T2 DM group, the fasting blood glucose was higher than 6.8 mmol/L, the serum insulin, leptin, and adiponectin levels were significantly decreased, and glucose intolerance and insulin resistance were measured as clinical indicators. Regarding pathological indicators, a large number of pancreatic islet cells showed the reduction of insulin secretion, resulting in damaged glycogen synthesis and liver steatosis. At the molecular level, the insulin signal transduction pathway was inhibited by decreasing the insulin receptor substrate 1 ( IRS 1), insulin receptor substrate 2 ( IRS 2), phosphatidylinositol 3 kinase ( PI 3K), and glycogen synthesis kinase 3β ( GSK ‐3β) expression levels. Conclusion The results show that the ZDF /T2 DM rats have typical clinical, histopathological, and molecular characteristics of human T2 DM and thus can be used as an effective model for T2 DM drug development and treatment of advanced T2 DM .