Open AccessAcute vitreoretinal trauma and inflammation after traumatic brain injury in mice
Author(s) -
Evans Lucy P.,
Newell Elizabeth A.,
Mahajan MaryAnn,
Tsang Stephen H.,
Ferguson Polly J.,
Mahoney Jolonda,
Hue Christopher D.,
Vogel Edward W.,
Morrison Barclay,
Arancio Ottavio,
Nichols Russell,
Bassuk Alexander G.,
Mahajan Vinit B.
Publication year - 2018
Publication title -
annals of clinical and translational neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.824
H-Index - 42
ISSN - 2328-9503
DOI - 10.1002/acn3.523
Subject(s) - medicine , traumatic brain injury , pathology , pathophysiology , retina , ophthalmology , eye injuries , poison control , neuroscience , injury prevention , biology , environmental health , psychiatry
Objective Limited attention has been given to ocular injuries associated with traumatic brain injury ( TBI ). The retina is an extension of the central nervous system and evaluation of ocular damage may offer a less‐invasive approach to gauge TBI severity and response to treatment. We aim to characterize acute changes in the mouse eye after exposure to two different models of TBI to assess the utility of eye damage as a surrogate to brain injury. Methods A model of blast TBI ( bTBI ) using a shock tube was compared to a lateral fluid percussion injury model ( LFPI ) using fluid pressure applied directly to the brain. Whole eyes were collected from mice 3 days post LFPI and 24 days post bTBI and were evaluated histologically using a hematoxylin and eosin stain. Results bTBI mice showed evidence of vitreous detachment in the posterior chamber in addition to vitreous hemorrhage with inflammatory cells. Subretinal hemorrhage, photoreceptor degeneration, and decreased cellularity in the retinal ganglion cell layer was also seen in bTBI mice. In contrast, eyes of LFPI mice showed evidence of anterior uveitis and subcapsular cataracts. Interpretation We demonstrated that variations in the type of TBI can result in drastically different phenotypic changes within the eye. As such, molecular and phenotypic changes in the eye following TBI may provide valuable information regarding the mechanism, severity, and ongoing pathophysiology of brain injury. Because vitreous samples are easily obtained, molecular changes within the eye could be utilized as biomarkers of TBI in human patients.