Open AccessCD8 + T cell subpopulations and pro‐inflammatory cytokines in neuromyelitis optica spectrum disorder
Author(s) -
Shi Ziyan,
Qiu Yuhan,
Zhao Zhengyang,
Wen Dingke,
Chen Hongxi,
Du Qin,
Zhang Ying,
Wang Jiancheng,
Yan Chao,
Yang Mu,
Zhou Hongyu
Publication year - 2021
Publication title -
annals of clinical and translational neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.824
H-Index - 42
ISSN - 2328-9503
DOI - 10.1002/acn3.51241
Subject(s) - cd8 , medicine , immunology , multiple sclerosis , neuromyelitis optica , interleukin 7 receptor , t cell , flow cytometry , immunotherapy , cytotoxic t cell , tumor necrosis factor alpha , il 2 receptor , immune system , biology , biochemistry , in vitro
Objective Our study aimed to investigate circulating CD8 + T cell subpopulations and pro‐inflammatory cytokines in the neuromyelitis optica spectrum disorder (NMOSD). Methods A total of 121 peripheral blood samples were obtained from 57 patients with NMOSD, 34 patients with multiple sclerosis (MS), and 30 sex‐ and age‐matched healthy controls (HCs) for detection of CD8 + T cell subpopulations, including phenotypes of naïve (T N , CD62L hi CD45RO ‐ ), effector/memory (T E/M , CD62L lo CD45RO + ), memory precursor (T MP , CD127 hi KLRG1 lo ), and short lived effector (T SLEC , CD127 lo KLRG1 hi ). In addition, 36 samples from 18 NMOSD, 12 MS, and 6 sex‐ and age‐matched HCs for detecting pro‐inflammatory cytokines (IFNγ and TNFα) using flow cytometry. Results Compared with HCs, we found significantly reduced CD8 + T N and increased CD8 + T E/M in both NMOSD and MSwhile decreased CD8 + T MP was only observed in NMOSD. Patients treated with immunotherapy were associated with increased CD8 + T N and decreased CD8 + T E/M in NMOSD. Moreover NMOSD cohort showed significant higher proportions of IFNγ + CD8 + T cells and proportions of TNFα + CD8 + T cells than HC and MS cohorts. On the contrary, obviously decreased IFNγ and TNFα were found in NMOSD patients treated with immunotherapy. Furthermore, Multivariate linear regression analyses revealed that age was negatively correlated with CD8 + T N and T MP , and positively associated with T SLEC ; however, sex, EDSS scores and disease phase were not significantly associated with CD8 + T subpopulations. Interpretation This current study provides an evidence that circulating CD8 + T cell with abnormal subpopulations and increased pro‐inflammatory were associated with pathogenesis of autoimmune demyelinating disease of CNS, especially in NMOSD.