Open AccessExtended and direct evaluation of RT ‐Qu IC assays for Creutzfeldt‐Jakob disease diagnosis
Author(s) -
Groveman Bradley R.,
Orrú Christina D.,
Hughson Andrew G.,
Bongianni Matilde,
Fiorini Michele,
Imperiale Daniele,
Ladogana Anna,
Pocchiari Maurizio,
Zanusso Gianluigi,
Caughey Byron
Publication year - 2017
Publication title -
annals of clinical and translational neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.824
H-Index - 42
ISSN - 2328-9503
DOI - 10.1002/acn3.378
Subject(s) - medicine , cerebrospinal fluid , disease , virology , pathology
Real‐Time Quaking‐Induced Conversion ( RT ‐Qu IC ) testing of human cerebrospinal fluid ( CSF ) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non‐prion disease patients, we provide the first direct and concurrent comparison of our improved RT ‐Qu IC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2‐day reduction in average detection time, and 100% specificity.