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Human TUBG 2 gene is expressed as two splice variant mRNA and involved in cell growth
Author(s) -
Ohashi Tsubasa,
Yamamoto Tadashi,
Yamanashi Yuji,
Ohsugi Miho
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12163
Subject(s) - gene , messenger rna , biology , splice , alternative splicing , gene expression , blot , microbiology and biotechnology , microtubule , cell growth , genetics
In mammals, γ‐tubulin, a key protein in microtubule nucleation, is encoded by two genes, TUBG 1 and TUBG 2 . Human TUBG 1 and TUBG 2 mRNA are expressed ubiquitously and predominantly in preimplantation embryos and the brain, respectively, but specific detection of γ‐tubulin2 protein expression is difficult due to their high sequence similarity. Here, we describe a protocol for differential detection of two human γ‐tubulins by western blotting. In several cancer cell lines and the brain, expression of γ‐tubulin2 along with γ‐tubulin1 and a novel TUBG 2 splice variant are identified. Contribution of ectopically expressed γ‐tubulin2 in cancer growth was determined by depletion of γ‐tubulin2.