Premium
Nuclear Drosophila CerS Schlank regulates lipid homeostasis via the homeodomain, independent of the lag1p motif
Author(s) -
Voelzmann André,
Wulf AnnaLena,
Eckardt Franka,
Thielisch Melanie,
Brondolin Mirco,
Pesch YaninaYasmin,
Sociale Mariangela,
Bauer Reinhard,
Hoch Michael
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12125
Subject(s) - homeobox , nuclear localization sequence , biology , microbiology and biotechnology , ceramide , mutant , nuclear transport , phenotype , lipid metabolism , genetics , gene , biochemistry , cell nucleus , nucleus , transcription factor , apoptosis
Drosophila Ceramide Synthase (CerS) Schlank regulates both ceramide synthesis and fat metabolism. Schlank contains a catalytic lag1p motif and, like many CerS in other species, a homeodomain of unknown function. Here, we show that the Drosophila CerS Schlank is imported into the nucleus and requires two nuclear localization signals ( NLS s) within its homeodomain and functional Importin‐β import machinery. Expression of Schlank variants containing the homeodomain without functional lag1p motif rescued the fat metabolism phenotype of schlank mutants whereas a variant with a mutated NLS site did not rescue. Thus, the homeodomain of Schlank is involved in the regulation of lipid metabolism independent of the catalytic lag1p motif.