English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Record-holding super-resolution microscopy (SRM) techniques, e.g.localization microscopy (LM) and stimulated-emission-depletion (STED)microscopy, require significant resources and effort from life-scienceresearchers. In comparison with confocal microscopy, apart from relying onextensive sample-staining procedures and long acquisition times, applying LMfor 3D and multicolor imaging poses a considerable experimental challenge. Inthe current work, we provide a complete demonstration of an entry-level SRMtechnique - providing super-resolving capabilities with an experimentalcomplexity level akin to that of confocal microscopy. Exchanging the confocalpinhole with a small pixelated detector, we use the inherent fluctuations ofdye molecules as contrast for SRM. This contrast is processed into asuper-resolved image through a process of pixel reassignment, a robust anddeterministic algorithm. Since the fluctuation contrast is ubiquitous toorganic markers, it does not require engineering of the blinking statisticsthrough the sample buffer. Together with the built-in capabilities formulti-color and 3D imaging, shown in our work, it can become a naturalextension to confocal microscopy - a straightforward first step into the realmof SRM.

Robust fluctuation-based super-resolution microscopy in a confocal architecture