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Wide-field imaging is widely adopted due to its fast acquisition,cost-effectiveness and ease of use. Its extension to direct volumetricapplications, however, is burdened by the trade-off between resolution anddepth of field (DOF), dictated by the numerical aperture of the system. Wedemonstrate that such trade-off is not intrinsic to wide-field imaging, butstems from the spatial incoherence of light: images obtained through spatiallycoherent illumination are shown to have resolution and DOF independent of thenumerical aperture. This fundamental discovery enabled us to demonstrate anoptimal combination of coherent resolution-DOF enhancement and incoherenttomographic sectioning for scanning-free, wide-field 3D microscopy on amulticolor histological section.

Direct 3D imaging through spatial coherence of light