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A novel technique for studying the demixing of binary fluids with nanosecondtime resolution at nanometer spatial resolution is demonstrated. Nanosecondkinetics of phase separation initial stages in the water/2,6-lutidine mixturestudied through the combination of a laser-induced temperature jump, andForster Resonant Excitation Transfer techniques. A pair of chromophores:8-hydroxy-1,3,6-pyrenetrisulfonate (HPTS) and Coumarin 480 form a FRET pair inthe uniform phase of the mixture and separate into the components of themixture in the nonuniform phase. The temperature jump that brings the mixtureinto a nonuniform phase is performed by the mid-infrared laser pulse. Theexponential growth stage of phase separation is in the 5 to 20 nanosecondsrange. The demonstrated technique can be applied to a broad range of chemicaland biological systems.

Nanosecond kinetics of phase separation in binary fluid studied by a  combination of Laser-induced temperature jump and Forster Resonant Excitation  Transfer probing